Optogenetic control
Engineering →A green-light switch built into B. subtilis.
- Four Level 1 modules built and sequenced
- Light array on 520 and 660 nm LEDs, six conditions
No induction run exists anywhere, so the switch is still design intent.
The big picture
Green light is meant to switch the cells on, the reactor keeps them in, and the protectant is what they are built to make. Each pair of them shares one mechanism, named between them. Point at anything to see what it touches.
A green-light switch built into B. subtilis.
No induction run exists anywhere, so the switch is still design intent.
Green light on one side, the vessel’s own telemetry at 1 Hz on the other. The reactor’s lamp is not built, and the loop has never closed.
A vessel that grows the cells and holds them.
The membrane and the photometer have never run as one machine.
One 0.2 µm pore holds the rods in and is wide enough to pass a 37.6 kDa enzyme. Shell fluid plated clean on 6 June; nothing has been measured crossing.
Three molecules: ACC deaminase, LEA14 and BoPep4.
No protectant has been measured leaving a cell, and the blot found none in the prep.
The promoter the light drives and the protectant it makes are one plasmid, sequenced with no mutations. It has never been induced.
The three parts are for this: a root that survives salt.
No protectant has rescued a plant yet.
Three parts are built and measured. One joint is real in DNA, one in hardware, and the third is still on paper: the reactor has no lamp of its own. Not one has been measured working, and a rust mark says where.